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Laboratory

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Our current laboratory capacity is suited for our role as a regional health center providing diagnostics services for primary care, obstetrics and gynecology, pediatrics, and emergency services.  This page provides a concise overview of the services that we currently provide; operations details are provided on our Laboratory Manual.  In addition to our laboratory, we have initiated a diagnostic imaging program to serve the diagnostic needs of our clinical team.  Additionally, this overview of our clinical programs provides a description of the services that our laboratory serves. 

 

As part of our overall task-shifting strategy, we are training midwives to perform certain basic laboratory tests.  These are marked with **stars.

 


Colorimetry-Based Tests

The colorimeter has a photometric settings of 450, 470, 510, 520, 540, 570, 600, and 670 nm.  This is adequate for most colorimetric applications.  The current tests include:

  • Hemoglobin (Drabin's), primarily for antenatal screening
  • AST (DNPH), primarily for evaluation of patients on antiretroviral/anti-tuberculosis therapy
  • ALT (DNPH), very occasional use among complex patients
  • Bilirubin (DMSO), primarily for triage and evaluation of neonatal jaundice
  • Creatinine (Jaff), primarily in triage of acutely ill patients
  • Glucose, Serum (Glucose Oxidase)
  • Albumin (BCG), occassional use in evaluation of malnutrition 

 

Routine Microscopy:

  • Gram Stain
  • Wet mount
  • KOH prep
  • Sputum for acid fast bacilli (integrated within Nepal government's national quality control system-- we send all positives and 10% of negatives to national lab) 

 

QBC AutoRead Plus Tests**:

  • hematocrit,
  • hemoglobin
  • mean corpuscular hemoglobin concentration,
  • platelet count
  • white blood cell count
  • granulocyte count and percentage
  • combined lymphocyte/monocyte count and percentage

 

Rapid Kit-Based Tests**:

  • RPR
  • HIV
  • Anti-ABD, pregnancy screening
  • Glucometer and cuvettes
  • Urine, protein/glucose, for use in antenatal screening
  • Urine, 9-panel, for use in UTI evaluation
  • Urine, beta-HCG for pregnancy 
  • Helicobacter pylori

 

Blood Electrolytes: i-STAT®1 analyser**

 

Equipment list at Bayalpata Hospital 

  • 1 light microscope
  • 1 fridge
  • 1 centrifuge
  • 1 photometer
  • 1 weighing scale
  • 1 QBC counter
  • 1 QBC centrifuge
  • 1 incubator (broken)
  • 1 I-STAT 
  • 1 VDRL rotator (broken)
  • 1 Paralens (advanced TB microscope, not currently being used due to lack of auramine stain)
  • 3 micropipettes

     

  • Desired Equipment
    • new light microscope
    • backup micropipettes
    • hot air oven for reagents
    • incubator

 

Expansion

As Nyaya Health expands its diagnostic capacity, the following testing systems will be additionally integrated into the laboratory services at the clinic:

  • Culture Microbiology
  • Additional colorimetry-based tests (amylase, lipid profile, CSF Protein, CSF Glucose)
  • Technician-based Microscopy (Stool examination, CSF cell count, thin blood smear, urine sediment microscopy)
  • Blood transfusion laboratory tests (HBsAg, HCV, DAT, IAT, RBC antibody, crossmatch)
  • Chemistry analyzer
  • Immunodiagnostics (e.g., Thyroid Function Tests) 

 

Additional Materials 

Guidelines for laboratory services:

http://www.phclab.com/images/MCH.pdf

http://www.phclab.com/images/Nepal%20SA.pdf

http://www.phclab.com/images/PHC.pdf

http://www.icddrb.org/images/HSB_Eng51_essential.pdf

--books: http://www.phclab.com/Resource%20Centre/RCbooks.htm

--documents: http://www.phclab.com/Resource%20Centre/RCdocuments.htm

--training: http://www.phclab.com/Training/Trainingprograms.htm

 

Other Links: 

District Laboratory Practice in Tropical Countries By Monica Cheesbrough

http://www.globalgiving.com/pfil/1651/projdoc.doc

http://jcp.bmj.com/cgi/content/full/58/1/56

http://members.tripod.com/~LouCaru/index-5.html

http://tropej.oxfordjournals.org/cgi/content/citation/38/2/50

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